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目的:通过光遗传学手段,特异性调控内侧前额叶皮质(medial prefrontal cortex,m PFC)锥体细胞活性的变化,观察对于大鼠内脏痛及焦虑行为的影响。方法:雄性成年大鼠分为3组,随机注入AAV_(2/9)-Ca MKII-mcherry,AAV_(2/9)-Ca MKII-ChR2-mcherry以及AAV_(2/9)-Ca MKII-Arch T-mcherry病毒,特异性感染锥体细胞。病毒注射4周后,将光纤埋入双侧m PFC内,之后通过给予蓝光或黄光刺激激活含ChR2或Arch T的神经元,观察对于大鼠内脏痛和焦虑行为的影响。结果:AAV注射很好地感染了m PFC内的锥体细胞,表达ChR2的大鼠经蓝光照射后Fos表达量增高,内脏痛反应和焦虑行为受到抑制。表达Arch T的大鼠经黄光刺激后Fos表达量降低,内脏痛反应和焦虑行为增加。结论:兴奋m PFC内的锥体细胞对于内脏痛和焦虑产生明显抑制,而抑制锥体细胞则增强内脏痛和焦虑行为。
OBJECTIVE: To investigate the effects on the visceral pain and anxiety in rats through the regulation of the activity of pyramidal cells in the medial prefrontal cortex (m PFC) by means of optogenetics. METHODS: Male adult rats were randomly divided into 3 groups: AAV_ (2/9) -Ca MKII-mcherry, AAV_ (2/9) -Ca MKII-ChR2-mcherry and AAV_ (2/9) -Ca MKII-Arch T-mcherry virus, specifically infected pyramidal cells. Four weeks after the virus injection, the fibers were embedded in bilateral m PFCs and then neurons containing ChR2 or Arch T were activated by blue or yellow light stimulation to observe the effects on visceral pain and anxiety in rats. RESULTS: AAV injection infected pyramidal cells in m PFC very well. The expression of Fos in ChR2-treated rats was increased after exposure to blue light, and visceral pain and anxiety were inhibited. Arch T-induced rat Fos expression decreased, visceral pain response and anxiety increased after yellow light stimulation. CONCLUSIONS: Excited pyramidal cells in m PFC significantly inhibit visceral pain and anxiety, whereas inhibition of pyramidal cells enhances visceral pain and anxiety.