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目的:评价和颜坤泰胶囊(HYKT)对体外小鼠卵巢颗粒细胞的毒性保护作用。方法:取原代培养的小鼠颗粒细胞,分别予不同浓度的多柔比星(DOXO)和去氧乙烯环己烯(VCD)诱发细胞毒性;设定高、中、低剂量(分别为16.7、8.35、4.18 mg/ml)坤泰胶囊组及空白对照组,药物干预后用四甲基偶氮唑盐比色法(MTT法)测定各组小鼠卵巢颗粒细胞的存活率。结果:空白组显示,DOXO及VCD作用下小鼠卵巢颗粒细胞的存活率分别为55%、33%左右;HYKT干预后,高、中、低剂量组小鼠卵巢颗粒细胞存活率均较空白组明显提高(P<0.05或P<0.01),其中对DOXO的保护作用呈剂量依存性,而对VCD无明显的剂量依存性。结论:和颜坤泰胶囊对DOXO和VCD所诱发的生殖毒性均有明显的保护作用。
Objective: To evaluate the toxic protective effect of Yankun Tai Capsule (HYKT) on ovarian granulosa cells in vitro. Methods: Primary cultured mouse granulosa cells were induced with different concentrations of doxorubicin (DOXO) and cyclohexene oxide (VCD) to induce cytotoxicity. High, medium and low doses (16.7 , 8.35 and 4.18 mg / ml respectively), KunTai capsule group and blank control group. The survival rate of ovarian granulosa cells of mice in each group was determined by MTT method after the drug intervention. Results: The blank group showed that the viability of ovarian granulosa cells in mice treated with DOXO and VCD were 55% and 33%, respectively. After HYKT intervention, the survival rates of ovarian granulosa cells in high, medium and low dose groups were higher than those in blank group (P <0.05 or P <0.01). The protective effect of DOXO was dose-dependent, but there was no dose-dependent dependence on VCD. Conclusion: Yan Kuntai capsule has obvious protective effect on reproductive toxicity induced by DOXO and VCD.