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串行晶体学作为一种解析蛋白质晶体结构的新方法,因其拥有室温采集、辐射损伤低、时间分辨等优势而得到迅速发展。早期发展于自由电子激光的串行飞秒晶体学已成功实现了蛋白质微小晶体结构的解析。为了引入串行晶体学的技术优势,充分发挥第三代同步辐射的丰富资源和特点,基于上海光源BL17U1生物大分子线站,我们对串行晶体学上样方法进行了理论和实验研究,解决了静电纺丝上样方式在同步辐射上应用的难题,用溶菌酶微小晶体论证了其可行性;同时探讨了基于同步辐射的串行晶体学在毫秒时间尺度上的发展潜力,为光束线技术升级提供参考。
As a new method for analyzing the crystal structure of protein, serial crystallography has been rapidly developed due to its advantages of acquisition at room temperature, low radiation damage and time resolution. The early development of free-electron laser serial femtosecond crystallography has been successfully realized the analysis of protein micro-crystal structure. In order to introduce the technical advantages of serial crystallography and give full play to the rich resources and characteristics of the third generation of synchrotron radiation, based on the Shanghai BL70U1 biomacromolecule station, we conducted a theoretical and experimental study on the serial crystallographic sampling method The feasibility of applying electrospinning method to synchrotron radiation was discussed. The feasibility of using the lysozyme microcrystal was demonstrated. The development potential of synchrotron radiation-based serial crystallography on the millisecond time scale was discussed. Upgrade for reference.