论文部分内容阅读
The α subunit of the stimulatory G protein, G_(sα), is involved in the stimulation of adenylate cyclase pathway of signal transduction. The various G_(sα) mRNAs are generated either by alternative splicing or by using alternative promoter. A novel aberrant splicing of G_(sα) transcript in human leukemia cell lines is reported. The entire coding region of G_(sα) gene obtained, referred to as G_(sαLeu), was amplified by PCR with cDNA from Jurkat human leukemia cDNA library or the reverse-transcribed first strand cDNA from human leukemia cell line HL-60 as a template. The result of DNA sequencing indicated that,compared with originally reported G_(sα), G_(sαLeu) has two in-frame deletions, one in amino acid residues 23—249 and the other in amino acid residues 254—260, suggesting that G(sαLeu) may play an important role in leukemia. The complete coding region of G_(sαLu) was inserted downstream of the thrombin site of pGEX-2T fusion protein expression vector and expressed in the form of GST/G_(s
The α subunit of the stimulatory G protein, G_(sα), is involved in the stimulation of adenylate cyclase pathway of signal transduction. The various G_(sα) mRNAs are generated either by alternative splicing or by using alternative promoter. A novel aberrant splicing The G_(sα) transcript in human leukemia cell lines is reported. The present coding region of G_(sα) gene obtained, referred to as G_(sαLeu), was amplified by PCR with cDNA from Jurkat human leukemia cDNA library or the reverse- The transcribed first strand cDNA from human leukemia cell line HL-60 as a template. The result of DNA sequencing indicated that,compared with originally reported G_(sα), G_(sαLeu) has two in-frame deletions, one in amino acid residues 23 249 and the other in amino acid residues 254-260, suggesting that G(sαLeu) may play an important role in leukemia. The complete coding region of G_(sαLu) was inserted downstream of the thrombin site of pGEX-2T fusion protein expression Vector and expressed in The form of GST/G_(s