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Objective: The purpose of this study was to investigate the presence and distribution of Hepatitis B virus antigen (HBVAg)- and Hepatitis B virus deoxyribonucleic acid (HBV DNA) in ovary with chronic hepatitis B virus. Study design: The immunohistochemistry method and in situ hybridization method were used to detect the hepatitis B surface antigen (HBsAg)- , anti- hepatitis B core antibody (HBcAg), and HBV DNA in ovary of patients with chronic hepatitis B infection. Results: HBsAg presented in the ovum- granular cell and interstitial cell of ovary. HBsAg located in the cytomembrane and cytoplasm. HBcAg presented in the ovum- granular cell- interstitial cell and endothelium cell of interstitial blood vessel of ovary, and the HBcAg may present in the ovum at different stage. HBcAg was situated at the cytomembrane, cytoplasm, and nucleus. HBV DNA was detected in the ovum at different stage as HBcAg and in granular cell, interstitial cell of ovary. In the same ovary tissue, HBV DNA was positive in some ova, but it was weakly positive or negative in other ova. HBV DNA distributed mainly in nucleus, but also can be detected in the cytoplasm. Conclusion: HBV could infect the ovum at different stage and replicate in it. This may be an important mechanism of HBV vertical transmission.
Objective: The purpose of this study was to investigate the presence and distribution of Hepatitis B virus antigen (HBVAg) - and Hepatitis B virus deoxyribonucleic acid (HBV DNA) in ovary with chronic hepatitis B virus. Study design: The immunohistochemistry method and in situ hybridization method were used to detect the hepatitis B surface antigen (HBsAg) -, anti-hepatitis B core antibody (HBcAg), and HBV DNA in ovary of patients with chronic hepatitis B infection. Results: HBsAg presented in the ovum- granular cell and HBsAg located in the cytomembrane and cytoplasm. HBcAg presented in the ovum- granular cell- interstitial cell and endothelium cell of interstitial blood vessel of ovary, and the HBcAg may present in the ovum at different stage. HBcAg was situated at the cytomembrane, cytoplasm, and nucleus. HBV DNA was detected in the ovum at different stages as HBcAg and in granular cells, interstitial cell of ovary. In the same ovary tissue, HBV DNA was pos itive in some ova, but it was weakly positive or negative in other ova. HBV DNA distributed mainly in nucleus, but also be detected in the cytoplasm. Conclusion: HBV could infect the ovum at different stage and replicate in it. an important mechanism of HBV vertical transmission.