论文部分内容阅读
目的:评估脑梗死患者对氧磷酶1(paraoxonase 1,PON1)的基因192G/A多态性与脑梗死间的关系。方法:采用聚合酶链反应-限制性片段长度多态性(PCR-RFLP)方法分析295例脑梗死患者(脑梗死组)及117名正常对照者(对照组)的PON1基因192G/A多态性。结果:PON1基因192G/A多态性在对照组和脑梗死组中的GG型、GA型和AA型基因型分布分别为36.75%、50.43%、12.82%和28.14%、50.85%、21.02%。G、A等位基因分布在对照组和脑梗死组中分别为61.97%、38.03%和53.56%、46.44%。经检验,PON1基因192G/A位点基因型频率在2组人群中的分布差异无统计学意义(P>0.05),而等位基因频率在2组人群中的分布差异有统计学意义(P<0.05),脑梗死组的等位基因A与对照组比较,其比数比为1.2210,95%可信区间(95%CI)为1.0147～1.4793。结论:PON1基因192G/A多态性可能与脑梗死有关。
Objective: To evaluate the relationship between 192G / A polymorphism of paraoxonase 1 (PON1) and cerebral infarction in patients with cerebral infarction. Methods: Polymerase chain reaction-restriction fragment length polymorphism (PCR-RFLP) was used to analyze the 192G / A polymorphism of PON1 gene in 295 patients with cerebral infarction (cerebral infarction group) and 117 healthy controls (control group) Sex. RESULTS: The 192G / A polymorphisms of PON1 gene were 36.75%, 50.43%, 12.82% and 28.14%, 50.85% and 21.02% in the control and cerebral infarction groups, respectively. The distribution of G and A alleles were 61.97%, 38.03% and 53.56% and 46.44% respectively in the control group and the cerebral infarction group. There was no significant difference in the frequency of 192G / A locus in PON1 gene between the two groups (P> 0.05), but there was significant difference in allele frequencies among the two groups (P <0.05). Compared with the control group, allele A in cerebral infarction group was 1.2210, 95% confidence interval (95% CI) was 1.0147 ~ 1.4793. Conclusion: The 192G / A polymorphism of PON1 gene may be related to cerebral infarction.