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斑茅割手密复合体(GXAS07-6-1)是广西蔗茅属斑茅和广西甘蔗属割手密的属间杂种,聚集了双亲的优点。本研究利用基于Alu-like的PCR鉴定方法对GXAS07-6-1及甘蔗与GXAS07-6-1的3份F_1材料进行真实性鉴定,基于基因组原位杂交技术(GISH)对父本GXAS07-6-1及其3份F_1染色体组成及核型进行分析。研究结果表明:3份F_1材料为GXAS07-6-1的真杂种;父本GXAS07-6-1的染色体众数为62条,其中30条来自蔗茅属斑茅,32条来自甘蔗属割手密,核型分类属于1B,其染色体按“n+n”方式传递;GXASF_108-2-17、GXASF_108-2-22、GXASF_108-2-32的染色体数目为78~80条,其中69~71条来自甘蔗属,9~11条来自蔗茅属斑茅,3份F_1的核型分类分别属于2B、1B、1B,染色体传递方式均为“n+n”。父本GXAS07-6-1及3份F_1材料中均未发现有染色体的交换或易位现象。甘蔗与斑割复合体杂交,蔗茅属斑茅染色体在亲子间传递过程存在丢失现象。
The hybrids (GXAS07-6-1) of Radix Cotyledon are the interspecific hybrid between the two cotyledons of Radix Cinnabarin in Guangxi and the cotyledons of Guangxi Cunninghamia. They gather the advantages of their parents. In this study, three F_1 materials of GXAS07-6-1, sugarcane and GXAS07-6-1 were identified by PCR-based Alu-like method. The authenticity of GXAS07-6 was determined based on the genomic in situ hybridization (GISH) -1 and its F_1 chromosome composition and karyotype analysis. The results showed that the three F1 hybrids were true hybrids of GXAS07-6-1, the chromosome number of male GXAS07-6-1 was 62, of which 30 came from Ctenocephala yunnanensis and 32 from Cercaris The chromosomes were classified as 1B and their chromosomes were transmitted by “n + n”. The chromosome numbers of GXASF_108-2-17, GXASF_108-2-22 and GXASF_108-2-32 ranged from 78 to 80, of which 69 ~ 71 from sugarcane, 9 to 11 from Ctenopharyngodon idellus, and 3 F_1 karyotypes belong to 2B, 1B and 1B, respectively. The chromosomal transmission patterns are both “n + n”. No chromosomal exchange or translocation was found in the male parent GXAS07-6-1 and the three F_1 materials. Sugarcane and plaque complex hybrids, Ctenocephala diantan chromosome transmission loss in the course of parent-child phenomenon.