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目的证实链脲佐菌素(STZ)单侧腹腔注射建立大鼠糖尿病性勃起功能障碍模型的可行性。方法 45只SD大鼠随机分为对照组(15只)和实验组(30只)。实验组大鼠腹腔注射STZ 60mg/kg,对照组大鼠腹腔注射相应量的柠檬酸钠一柠檬酸缓冲液,3d后测血糖确定是否成模。此后,每周测血糖1次,8周后用阿朴吗啡(APO)80 μ g/kg颈部皮下注射,观察大鼠阴茎勃起情况。结果实验组糖尿病成模率为93.3%(28/30),在成模后8周的观察期间大鼠的死亡率为7.1%(2/28)。实验组血糖明显高于对照组(P<0.01),体重、阴茎勃起次数、阴茎勃起率则明显低于对照组,两组间差异有统计学意义(P<0.01)。结论 STZ 60mg/kg单侧腹腔内注射能安全有效地建立大鼠糖尿病模型;颈部皮下注射APO 80 μg/kg可有效地筛选出大鼠糖尿病勃起功能障碍。
Objective To confirm the feasibility of streptozotocin (STZ) unilateral intraperitoneal injection to establish diabetic erectile dysfunction model in rats. Methods 45 SD rats were randomly divided into control group (n = 15) and experimental group (n = 30). Rats in the experimental group were intraperitoneally injected with STZ 60mg / kg. The control group rats were intraperitoneally injected with the corresponding amount of sodium citrate-citrate buffer, and the blood glucose was determined after 3 days to determine whether they were modeled. Thereafter, blood glucose was measured once a week, and after 8 weeks, subcutaneous injections of 80 μg / kg apomorphine (APO) into the neck were used to observe the penile erection in rats. Results The rate of diabetes mellitus was 93.3% (28/30) in experimental group and 7.1% (2/28) in the observation period of 8 weeks after injection. The blood glucose in the experimental group was significantly higher than that in the control group (P <0.01). The body weight, number of erections and erection rate were significantly lower than those in the control group. There was significant difference between the two groups (P <0.01). Conclusion STZ 60mg / kg unilateral intraperitoneal injection can be safely and effectively established rat model of diabetes; subcutaneous injection of APO 80μg / kg in the neck can effectively filter out diabetic erectile dysfunction.