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目的:采用HPLC法对不同采收期的养心草药材进行指纹图谱研究,为养心草药材质量控制提供依据。方法:采用DiamonsilC18色谱柱(4.6mm×250mm,5μm),以乙腈-0.5%磷酸水为流动相进行梯度洗脱,流速1.0mL∕min,柱温25℃,检测波长254nm。结果:以21个共有峰为评价指标,精密度和重复性实验中各共有峰相对保留时间和相对峰面积的RSD均小于3%,10批样品相似度大于0.9。结论:此方法精密度、稳定性和重复性良好,有助于养心草药材的质量控制。
OBJECTIVE: To study the fingerprinting of Yangsan herbs with different harvesting periods by HPLC to provide basis for the quality control of Yangsan herbs. METHODS: Diamonsil C18 column (4.6 mm × 250 mm, 5 μm) was used with gradient elution with acetonitrile-0.5% phosphoric acid as mobile phase at a flow rate of 1.0 mL / min and a column temperature of 25 ° C. The detection wavelength was 254 nm. Results: With 21 common peaks as the evaluation index, the RSDs of the relative retention time and the relative peak area of each common peak in the precision and repeatability experiments were less than 3%, and the similarity of 10 batches of samples was greater than 0.9. Conclusion: The precision, stability and repeatability of this method are good, which is helpful for the quality control of Yangxincao herbs.