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目的:探讨微囊藻毒素-LR对小鼠肝、肾和睾丸组织蛋白质的氧化损伤程度。方法:用3.0、6.0和12.0μg/kg3种不同浓度的微囊藻毒素-LR对小鼠进行腹腔注射染毒(n=5),染毒时间为7d,每天1次,并设阴性对照组。用2,4-二硝基苯肼比色法测定3种组织的蛋白质羰基含量。结果:随着微囊藻毒素-LR剂量的升高,小鼠肝、肾和睾丸的蛋白质羰基含量升高(F分别为44.631、21.975和23.962,P均〈0.001)。3.0μg/kg染毒组肝、肾蛋白质羰基含量[(3.72±0.23)和(3.99±0.48)mmol/kg]高于阴性对照组[(2.90±0.22)和(3.27±0.23)mmol/kg](P均〈0.05)。3.0μg/kg染毒组睾丸蛋白质羰基含量[(1.91±0.25)mmol/kg]与阴性对照组[(1.69±0.27)mmol/kg]相比,差异无统计学意义(P〉0.05)。6.0μg/kg染毒组肝、肾和睾丸蛋白质羰基含量[(4.55±0.45)、(4.44±0.53)和(2.53±0.24)mmol/kg]和12.0μg/kg染毒组肝、肾和睾丸蛋白质羰基含量[(5.62±0.55)、(5.67±0.60)和(3.15±0.41)mmol/kg]均高于阴性对照组(P均〈0.01)。结论:微囊藻毒素-LR对小鼠肝、肾和睾丸组织的蛋白质有氧化损伤作用。“,”Aim:To expore the degree of protein oxidative damage on liver, kindey and testicle of mice induced by microcystin-LR.Methods:A total of 20 mice were intraperitoneally administered microcystin-LR at doses of 0,3.0, 6.0 or 12.0 μg/kg body weight every day for 7 days.The protein carbonyl content in liver,kidney and testicle tissue was measured using spectrophotometric DNPH assay to reflect the degree of protein oxidative damage.Results:With the increase of microcystin-LR concentration protein carbonyl content in three kinds of tissue also increased(F was 44.631, 21.975 and 23.962,P0.001). Protein carbonyl contents of liver and kidney in 3.0 μg/kg microcystin-LR exposure group [(3.72±0.23) and (3.99±0.48) mmol/kg] were higher than those of control group [(2.90±0.22) and (3.27±0.23) mmol/kg] (P0.05). There was no significant difference in protein carbonyl content of testicle between 3.0 μg/kg microcystin-LR exposure group [(1.91±0.25) mmol/kg] and control group [(1.69±0.27) mmol/kg] (P0.05). Protein carbonyl content of liver, kidney,and testicle in 6.0 μg/kg microcystin-LR exposure group [(4.55±0.45), (4.44±0.53), (2.53±0.24) mmol/kg] and 12.0 μg/kg microcystin-LR exposure group [(5.62±0.55), (5.67±0.60),(3.15±0.41) mmol/kg] were higher than those of control group(P0.01).Conclusion: Microcystin-LR could induce protein oxidative damage of liver, kidney and testicle in mice.