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【目的】对26株迟缓爱德华氏菌进行自动化核糖体分型,并进行聚类分析。【方法】采用RiboprinterTM全自动微生物鉴定系统对分离自斑点叉尾鮰、日本鳗、多宝鱼、比目鱼、斑醴等宿主体内的迟缓爱德华氏菌进行核糖体分型,以限制性内切酶Eco RⅠ处理、切割菌株DNA;运用Bio Numerics软件分析图像数据。【结果】迟缓爱德华氏菌核糖体图谱与数据库中已有信息进行比对,ATCC15947和BYK00685的比对相似值>0.85,分别为0.95及0.90。条形码经软件分析共产生21种核糖体条带,聚类分析分为3个群。条带之间呈现明显的地域性差异和宿主差别。来自北方及南方的菌株除少数几株以外,各自聚集为一个群;所有人源株则分布在第三群。【结论】自动化核糖体分型可以方便快捷地用于不同物种的菌株分型与流行病学追踪溯源。
【Objective】 The objective of this study was to automate the ribosome typing of 26 strains of R. edwardsii and to perform cluster analysis. 【Method】 The RiboprinterTM automatic microbial identification system was used to perform ribosome typing of B. edwardsianus isolated from the host of Ictalurus punctatus, Japanese eel, polyp, halibut, spot sweet potato and the like, and restriction endonuclease Eco RⅠ , Strain DNA was cut; image data was analyzed using Bio Numerics software. 【Results】 The sequence of ribosome of L. edwardsiana was compared with the existing data in the database. The similarity of ATCC15947 and BYK00685 was> 0.85, respectively, 0.95 and 0.90. A total of 21 ribosome bands were generated by software analysis. The cluster analysis was divided into 3 groups. Bands showed obvious regional differences and host differences. In addition to a few strains, strains from the north and south clustered into one group each; all human strains were located in the third group. 【Conclusion】 Automated ribosome typing can be used conveniently and quickly for typing and epidemiological tracing of different species.