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用ＰＣＲ和ＥＬＩＳＡ两种方法同时检测了３９７例含有ＨＢＶ·ＤＮＡ免疫标志物一项以上阳性的乙肝病人血清和５０例正常人血清。结果在３９７例具有一项以上阳性的ＨＢＶ免疫标志物血清中，有１８６例在ＰＣＲ法检出ＨＢＶ·ＤＮＡ阳性，为４６．９％。正常人全部为阴性。按ＨＢＳＡｇ阴性、阳性分组，则ＨＢＳＡｇ阳性２９９例，ＰＣＲ检出ＨＢＶ·ＤＮＡ阳性１７１例，阳性率为５７．２％，ＨＢＳＡｇ阴性９８例，ＰＣＲ检出ＨＢＶ·ＤＮＡ阳性１５例，阳性率为１５．３％。说明ＰＣＲ法是检出ＨＢＶ·ＤＮＡ最灵敏、最特异的检测方法，是判断传染性简单、可靠的指标。是目前对微量ＨＢＶ·ＤＮＡ检测最先进的技术。但是还不能代替血清免疫指标的检测。
Three hundred and seventy-seven serum samples from hepatitis B patients and one hundred normal individuals who contained one or more HBV DNA markers were simultaneously detected by PCR and ELISA. Results Of the 397 serum HBV markers with more than one positive, 186 were positive for HBV DNA by PCR, accounting for 46.9%. Normal people are all negative. According to the positive and negative HBSAg group, 299 cases were positive for HBSAg. The positive rate of HBV DNA was 171 cases (57.2%) and 98 cases were negative for HBSAg (15 cases). The positive rate of HBV DNA was 15 .3%. PCR method is the detection of HBV DNA the most sensitive and most specific detection method is to determine the infectious simple and reliable indicator. It is the most advanced technology for the detection of trace HBV DNA. But it can not replace the detection of serum immune index.