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用含 0 0 2mol/LMES的 1mol/L盐酸胍抽提新鲜中国姥鲨软骨组织 ,抽提物经Mr30 0 0 0PTTK膜超滤、SephacrylS 2 0 0和Superdex 75柱层析获得 1种新的Mr 为 15 2 0 0的高纯度蛋白质 (Sp15 ) ,N 末端序列测定表明其与多种哺乳动物来源的溶菌酶具有相关性 ,并在体外具有显著的溶菌酶活性 ,其比活性高达 2 2 30 0 0U/mg ;Sp15在 10 μg/ml浓度下 ,可显著抑制血管内皮细胞的增殖 ,其抑制率高达 75 % ;在 8mg/kg剂量下 ,对小鼠移植的S180肉瘤的抑瘤率达 33 3% ,并可显著延长荷S180腹水瘤小鼠的生存时间。
A fresh Chinese basking Shark cartilage was extracted with 1 mol / L HCl containing 0 0 2 mol / LMES. The extract was purified by ultrafiltration using Mr30 0 0 PTTK membrane, and Sephacryls 2000 and Superdex 75 column chromatography. (15) was high purity protein (Sp15), N-terminal sequence analysis showed that it has a variety of mammalian lysozyme and has a significant in vitro lysozyme activity, its specific activity as high as 2 2 30 0 0U / mg; Sp15 could significantly inhibit the proliferation of vascular endothelial cells at a concentration of 10 μg / ml with the inhibition rate as high as 75%; at 8 mg / kg, the inhibition rate of S180 sarcoma in mice was 33 3 %, And can significantly prolong the survival of mice bearing S180 ascites tumor.