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以69份玉米自交系为试材,于温室模拟干旱胁迫环境,对各自交系的超氧化物歧化酶(SOD)活性、丙二醛(MDA)含量、脯氨酸(Pro)含量、相对电导率(REC)和相对含水量(RWC)等5项生理生化指标和茎粗、根冠比2项形态指标进行测定。通过对5项生理生化指标的耐旱系数的聚类分级,综合评价自交系的耐旱性。同时分析实验室条件下综合评价结果与形态指标鉴定结果的吻合度。结果表明:(1)受到胁迫时,材料的耐旱分级各不相同,材料间的响应机制各不相同;(2)筛选出耐旱性极强自交系4份、耐旱性强系14份、耐旱性中度系35份、耐旱性弱系12份、耐旱性极弱系4份;(3)根冠比、茎粗与综合评价结果吻合度低,不适宜作为实验室条件下玉米耐旱性的鉴定指标。
69 maize inbred lines were used as materials to simulate the drought stress environment in the greenhouse. The activities of superoxide dismutase (SOD), malondialdehyde (MDA), proline (Pro) (REC) and relative water content (RWC) and other five physiological and biochemical indicators and stem diameter, root and shoot ratio of 2 morphological indicators were measured. The drought tolerance of five inbred lines was evaluated comprehensively by clustering and grading the drought tolerance coefficients of five physiological and biochemical indexes. At the same time, analyze the concordance between the result of comprehensive evaluation and the result of morphological index under laboratory conditions. The results showed that: (1) Drought-tolerant grades of materials were different under different stresses, and the response mechanisms varied among different materials; (2) Four drought-resistant inbred lines , 35 moderately drought-tolerant lines, 12 weakly weakened lines and 4 very weak lines of drought tolerance. (3) The root-shoot ratio and stem diameter were not suitable for the laboratory as a result of the comprehensive evaluation Drought Tolerance Identification Index of Maize under Conditions.