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目的:应用生物信息学分析方法,阐述石榴皮对非酒精性脂肪肝病(NAFLD)的基因表达调控机制。方法:运用生物信息学分析工具QOE、DAVID和STRING,对NCBI的GEO数据库中石榴皮对NAFLD可能治疗作用的相关数据进行挖掘分析。结果:分析喂养与未喂养石榴皮的高脂饮食大鼠基因芯片数据,筛选出8个差异表达基因。其中包括7个高表达基因:CAD、C1QBP、RPL10、RPS7、NHP2、CARM1、LPL;1个低表达基因:NR3C1。这些基因主要涉及的功能包括:嘧啶核酸的生物合成、RNA的修复与加工、核糖体的生物合成、核糖核蛋白复合体、细胞质和细胞器重要物质、脂蛋白生物功能相关基因等。结论:石榴皮可能通过上调LPL、CARM1、C1QBP、NHP2、RPL10、RPS7、CAD和下调NR3C1基因表达的分子机制,促进甘油三酯(TG)的水解,降低血液中游离脂肪酸的含量,缓解胰岛素抵抗,增强肝细胞的抗氧化应激能力及维持线粒体功能的稳定,进而维持肝细胞的正常生物代谢,从而对NAFLD具有一定的疗效。
OBJECTIVE: To elucidate the regulatory mechanism of pomegranate peel on gene expression in non-alcoholic fatty liver disease (NAFLD) by bioinformatics analysis. METHODS: The bioinformatics tools QOE, DAVID and STRING were used to analyze the data of possible treatment effects of pomegranate peel on NAFLD in NCBI GEO database. Results: The gene chip data of high-fat diet rats fed with or without pomegranate bark were analyzed, and 8 differentially expressed genes were screened out. Including seven highly expressed genes: CAD, C1QBP, RPL10, RPS7, NHP2, CARM1, LPL; and a low expression gene: NR3C1. The main functions involved in these genes include: pyrimidine nucleic acid biosynthesis, RNA repair and processing, ribosomal biosynthesis, ribonucleoprotein complexes, cytoplasmic and organelles important substances, lipoprotein bio-function related genes. CONCLUSION: Pomegranate bark may promote the hydrolysis of triglyceride (TG), decrease the content of free fatty acids in blood and relieve insulin resistance by up-regulating the expression of LPL, CARM1, C1QBP, NHP2, RPL10, RPS7, CAD and down-regulating NR3C1 gene expression , Enhance the anti-oxidative stress ability of hepatocytes and maintain the stability of mitochondrial function, thus maintaining the normal biological metabolism of hepatocytes, thus having a certain effect on NAFLD.