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目的提取分离强心Ⅰ号方强心作用的有效部位。方法以大孔吸附树脂为分离材料,将强心Ⅰ号方组成药材的水提物、醇提物分别经蒸馏水,10%乙醇,30%乙醇,50%乙醇,70%乙醇,95%乙醇洗脱制得样品,然后通过离体蛙心试验观察受试样品的强心作用。结果水提物经30%乙醇,50%乙醇洗脱的样品和醇提物经30%乙醇,50%乙醇洗脱的样品对离体蛙心作用明显,其强心作用的半数有效浓度分别为:0.017g生药/m l,0.58 g生药/m l,10.11 g生药/m l,21.44 g生药/m l。结论强心Ⅰ号方中各味药材的水提物经30%乙醇,50%乙醇洗脱的样品和醇提物经30%乙醇,50%乙醇洗脱的样品具有明显强心作用。
Objective To extract the effective site of Qiangqiang I’s cardiac function. Methods The macroporous resin was used as the separation material. The aqueous extracts and alcohol extracts of Qiangxin I were made from distilled water, distilled water, 10% ethanol, 30% ethanol, 50% ethanol, 70% ethanol and 95% ethanol. Samples were prepared and then the cardiac effects of the test samples were observed by ex vivo frog heart tests. Results The samples of the aqueous extract eluted with 30% ethanol and 50% ethanol and the ethanol extracts eluted with 30% ethanol and 50% ethanol had obvious effects on the isolated frog heart, and the half effective concentrations of the cardiac effect were: : 0.017g crude drug/ml, 0.58g crude drug/ml, 10.11g crude drug/ml, 21.44g crude drug/ml. [Conclusion] The water extracts of each medicinal herb of Qiangxin I are eluted with 30% ethanol, 50% ethanol and ethanol extracts with 30% ethanol and 50% ethanol.