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目的研究丙型肝炎病毒(HCV)1b型5’非编码区BamHⅠ酶切位点变异的区域性分布。方法对来自中国4个不同地区的64例HCV1b基因型血清样品进行5’-NCR扩增后,分别用MboⅠ、BamHⅠ限制性内切酶酶切分析,比较不同地区变异株分布的差异。结果测序证实中国HCV1b基因型在117位有BamHⅠ酶切位点。64例血清样品中,BamHⅠ单切点变异株,华南7例(28%),东北1例(10%),华北1例(11.1%),西南0例;MboⅠ及BamHⅠ切点均无的变异株,西南8例(40%),华南2例(4%),东北1例(10%),华北0例。结论证实HCV1b型BamHⅠ变异具有区域性分布特点,华南地区和西南地区变异较多,华北和东北变异较少。而这一变异是否与干扰素治疗耐药有关,尚有待于进一步研究。
Objective To study the regional distribution of BamH Ⅰ restriction site mutation in the 5 ’non-coding region of hepatitis C virus (HCV) type 1b. Methods Sixty-four samples of HCV1b genotypes from four different regions in China were amplified by 5’-NCR and digested with restriction endonucleases MboⅠ and BamHⅠ to compare the differences in the distribution of the mutants in different regions. Results Sequencing confirmed that the HCV1b genotype in China had a BamHI restriction site at position 117. In 64 serum samples, BamH I single-site mutation was found in 7 cases (28%) in South China, 1 case (10%) in Northeast China, 1 case (11.1%) in North China and 0 cases in Southwest China 8 cases (40%) in Southwest China, 2 cases (4%) in South China, 1 case (10%) in Northeast China and 0 case in North China. Conclusion It is confirmed that the variation of BamHⅠ in HCV1b is characterized by regional distribution, with more variations in South China and Southwest China and less variation in North China and Northeast China. Whether this mutation is related to the resistance to interferon treatment remains to be further studied.