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目的探讨生物可降解药物丝裂霉素磁性纳米球(MMC-MNS)的释药特点,及其体内外抑制乳腺癌细胞生长的活性和特征。方法采用动态透析结合紫外分光光度法测定MMC- MNS在体外中性介质中的释药特性；以乳癌细胞株MCF-7为对象,SRB法测定MMC-MNS在4种不同药物浓度下的体外抑瘤效率；将乳腺癌皮下移植瘤裸鼠模型分为4组(分别为尾静脉注射生理盐水、MMC、空白MNS以及MMC-MNS组),瘤体表面均给予相同三维立体梯度磁场作用,观察MMC-MNS靶向治疗裸鼠乳腺癌的效果。结果MMC-MNS具有良好的缓释功能；MMC-MNS对乳癌细胞MCF-7有明显的杀伤活性,呈剂量-效应关系；经MMC-MNS处理后的肿瘤生长较其他组明显减慢,瘤重抑瘤率高达54．2%。结论MMC-MNS在体外有明显的药物缓释效应,能在较长时间维持有效作用浓度,在体内外均表现出良好的抗癌杀瘤作用,具有良好的临床抗肿瘤应用前景。
Objective To investigate the drug release characteristics of biodegradable drug mitomycin C magnetic nanospheres (MMC-MNS) and its activity and characteristics in inhibiting the growth of breast cancer cells in vitro and in vivo. Methods Dynamic dialysis combined with UV spectrophotometry was used to determine the drug release characteristics of MMC-MNS in neutral medium. Breast cancer cell line MCF-7 was used as the test substance. SMB assay was used to determine the in vitro inhibition of MMC-MNS at 4 different drug concentrations. Tumor efficiency; The nude mice model of subcutaneous xenografts of breast cancer was divided into 4 groups (intravenous saline, MMC, blank MNS, and MMC-MNS groups, respectively). The surface of the tumors were all given the same three-dimensional gradient magnetic field to observe the MMCs. - MNS-targeted treatment of breast cancer in nude mice. Results MMC-MNS had a good sustained-release function; MMC-MNS had a significant killing activity on breast cancer cell MCF-7 in a dose-effect relationship; tumor growth after MMC-MNS treatment was significantly slower than that of other groups, and the tumor weight was significantly lower. The tumor inhibition rate is as high as 54.2%. Conclusion MMC-MNS has obvious drug release effect in vitro and can maintain effective concentration for a long time. It shows good anti-cancer antitumor effect both in vitro and in vivo. It has a good clinical anti-tumor application prospect.