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目的 :探讨用干扰素治疗膀胱肿瘤时对膀胱肿瘤细胞膜肿瘤坏死因子受体 (TNFR)表达的影响。方法 :用放射配基结合分析法检测 α和 γ干扰素分别作用于膀胱癌 BIU- 87细胞前后细胞膜 TNFR的变化。结果 :膀胱肿瘤细胞膜存在 TNFR,受体的特异性结合量与干扰素浓度和作用时间关系密切 ;γ干扰素对受体表达的影响大于α干扰素。结论 :干扰素可以使膀胱肿瘤细胞膜 TNFR的表达增加 ,从而使免疫治疗后高表达的 TNF可以与肿瘤细胞膜受体更好地结合 ,以产生细胞毒作用 ,杀灭肿瘤细胞。
Objective: To investigate the effect of interferon on the expression of tumor necrosis factor receptor (TNFR) in bladder tumor cells. Methods: Radioimmunoassay was used to detect the changes of TNFR in the cell membrane of human bladder cancer cell line BIU-87 after treatment with α and γ interferon respectively. Results: There was TNFR in the cell membrane of bladder tumor. The specific binding of receptor was closely related to the concentration of interferon and the duration of action. The effect of IFN-γ on receptor expression was greater than that of IFN-α. CONCLUSION: Interferon can increase the expression of TNFR in the bladder tumor cell membrane, so that the highly expressed TNF after immunotherapy can better bind with the tumor cell membrane receptor to produce cytotoxicity and kill the tumor cells.