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近年来有许多实验室用遗传工程技术和体细胞诱变方法制备新一代单克隆抗体McAb,包括嵌合抗体、酶抗体和具有新型功能的抗体等,获得了初步成果。这种抗体将更有效地用于体内诊断、治疗疾病、提高免疫化学分析及有助于分析研究抗原抗体相互作用的机理。一、基本原理免疫球蛋白分子Ig是由两条相同的重链(H)和两条相同的轻链(L)组成的糖蛋白。每条链又分为恒定区(C_H、C_L)和可变区(V_H,V_L),V_H、V_L决定Ig结合抗原
In recent years, there are many laboratories using genetic engineering techniques and somatic mutagenesis methods to prepare a new generation of monoclonal antibodies McAbs, including chimeric antibodies, enzyme antibodies and antibodies with novel functions, obtained preliminary results. Such antibodies will be more effective for in vivo diagnosis, treatment of disease, enhancement of immunochemical chemistry and analysis of mechanisms of antigen-antibody interaction. I. Rationale Immunoglobulin Molecules Ig is a glycoprotein consisting of two identical heavy chains (H) and two identical light chains (L). Each chain is divided into constant area (C_H, C_L) and variable area (V_H, V_L), V_H, V_L determine Ig binding antigen