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应用闪光视网膜电图(fERG)、细胞色素c氧化酶(CO)组织化学染色及突触囊泡素免疫荧光染色方法检测视神经切断术后,视网膜外网层相关神经元的功能活动及突触可塑性变化。结果显示:fERGb波峰潜伏期在视神经损伤后均显著延长;b波振幅在损伤第3、5d组增高,此后逐渐下降并在7、14、21d组低于正常。外网层CO活性在视神经切断第3、5d上调,此后逐渐下降,至第14、21d低于正常。外网层突触囊泡素阳性颗粒在视神经切断第5d增多,此后逐渐下降,至第14、21d低于正常。本研究结果提示视神经切断后,视网膜内外网层神经元的功能在早期将出现一过性的增强,结构也有相应的代偿性改变,随后即发生跨神经元的逆行性溃变。
The functional activity and synaptic plasticity of retinal extracellular layer-related neurons were detected by flash electroretinogram (fERG), cytochrome c oxidase (CO) histochemical staining and immunocytochemical staining of synaptophysin Variety. The results showed that the latency of fERGb peak was significantly prolonged after optic nerve injury. The amplitude of b wave increased in the first and third day of injury, then decreased gradually and was lower than normal in the 7th, 14th and 21st day. Outer layer of CO activity in the optic nerve cut up 3,5d up, then gradually decreased to the 14th, 21d below normal. Extraocular layer of synaptophysin-positive particles in the optic nerve cut off 5d increased, then gradually decreased to the 14th, 21d below normal. The results suggest that the function of neurons in the inner and outer retinal layers of the retina will be transiently enhanced in the early stage after the optic nerve is cut off, and the structures also have corresponding compensatory changes, and subsequent retrograde neuronal degeneration occurs.