初步鉴定某种化学物是否为致突变物或致癌物,Ames等快速细菌试验是非常有效的手段。该法简便、灵敏和准确,既可检测硷基置换型,也可检测移码型回复突变的DNA损伤,并以哺乳动物肝或其它器官的微粒体制品为辅佐,使前致癌物能够活化为终致癌物的形式。目前世界上有数以千计的实验室采用该法来筛选环境来源的大量潜在致突变物和致癌物。 Ames试验的基本原理是用致突变物处 Preliminary identification of a chemical as a mutagen or carcinogen, Ames and other rapid bacterial test is a very effective means. The method is simple, sensitive and accurate. It not only detects base substitutions, but also detects DNA damage caused by frameshift mutation. In addition, it can be activated by microsomal preparations of mammalian liver or other organs The final carcinogen form. Thousands of laboratories around the world use this method to screen a large number of potential mutagens and carcinogens from environmental sources. The basic principle of Ames test is to use mutagens