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目的探讨PTEN、PKB在子宫内膜癌发病过程中的作用。方法应用逆转录聚合酶链反应 (RT-PCR)技术检测55例子宫内膜癌组织,13例子宫内膜非典型增生,11例增殖期内膜中PTEN及PKB mRNA的表达。结果 PTEN mRNA在正常内膜、非典型增生内膜及子宫内膜癌组织间表达阳性率分别为 81．8％、38．5％及36．4％,三者比较有显著性差异(P<0．05),而PKB mRNA在正常内膜、非典型增生及子宫内膜癌中表达阳性率为27．3％、46．2％及61．8％,但无统计学差异(P>0．05);在子宫内膜癌不同临床分期、病理分级之间,PTEN mRNA和PKB mRNA表达量均没有显著差异(P>0．05)。结论 PTEN可能通过PI3K- PKB途径导致子宫内膜癌的发生。
Objective To investigate the role of PTEN and PKB in the pathogenesis of endometrial cancer. Methods Reverse transcription-polymerase chain reaction (RT-PCR) was used to detect the expression of PTEN and PKB mRNA in 55 cases of endometrial cancer, 13 cases of endometrial dysplasia and 11 cases of proliferative endometrium. Results The positive rates of PTEN mRNA in normal endometrium, atypical endometrial and endometrial carcinoma tissues were 81.8%, 38.5% and 36.4%, respectively (P < 0.05). However, the positive rates of PKB mRNA expression in normal endometrium, atypical hyperplasia and endometrial carcinoma were 27.3%, 46.2% and 61.8%, respectively, but there was no significant difference (P> 0 .05). There was no significant difference in the expression of PTEN mRNA and PKB mRNA in different clinical stage and pathological grade of endometrial carcinoma (P> 0.05). Conclusions PTEN may cause endometrial cancer through the PI3K-PKB pathway.