论文部分内容阅读
背景:目前临床上虽有多种方法用于治疗软骨缺损,但没有从根本上解决关节软骨缺损修复问题。目的:通过组织学研究进一步评价柚皮苷结合组织工程软骨修复兔关节软骨缺损的效果。方法:取兔骨髓间充质干细胞体外增殖后,复合于改建后的脱细胞真皮基质载体上,制成组织工程软骨,植入到兔膝关节软骨缺损,并以柚皮苷汤灌胃,于4,8周后分别对修复组织进行苏木精-伊红、Masson三色染色、甲苯胺蓝染色、Ⅱ型胶原染色、Ⅹ型胶原染色等组织学检查。结果与结论:术后8周,柚皮苷结合干细胞复合体组缺损处修复组织变成乳白色,半透明光滑组织,缺损修复组织与周围正常软骨已基本难区分,表面光滑。组织学检查发现修复缺损处基本为新生软骨填充。结果证实,柚皮苷结合组织工程软骨能提高家兔膝关节软骨缺损的修复质量。
BACKGROUND: At present, although there are many ways to treat cartilage defects clinically, there is no fundamental solution to the problem of articular cartilage defect repair. OBJECTIVE: To further evaluate the effect of naringin combined with tissue-engineered cartilage in the repair of rabbit articular cartilage defect through histological study. METHODS: Rabbit bone marrow mesenchymal stem cells were proliferated in vitro and then combined with the modified acellular dermal matrix carrier to prepare tissue-engineered cartilage. The cartilage defects were implanted into rabbit knee articular cartilage defects and fed with naringin soup Four and eight weeks later, the histological examination of the repaired tissue was carried out by hematoxylin-eosin, Masson’s trichrome staining, toluidine blue staining, type II collagen staining and type X collagen staining. RESULTS AND CONCLUSION: At 8 weeks after operation, the tissue repaired by naringin combined with stem cell complex became milky white and translucent smooth tissue. The defect repair tissue was basically indistinguishable from the surrounding normal cartilage and had a smooth surface. Histological examination found that the repair defect is basically new cartilage filling. The results confirmed that naringin combined with tissue engineering cartilage can improve the repair quality of rabbit knee cartilage defects.