论文部分内容阅读
目的:观察RNA干扰靶向沉默hyrdC基因对胃癌SGC-7901细胞生长的影响。方法:将已成功构建的携带hyrdC基因以及沉默hyrdC基因的重组腺病毒Ad-hyrdC、Ad-shyrdC分别转染胃癌SGC-7901细胞,台盼蓝细胞计数和MTT法观察各组胃癌细胞生长情况,将胃癌细胞接种裸鼠皮下构建裸鼠荷瘤模型观察各组胃癌瘤体的生长情况。结果:Ad-shyrdC组细胞生长明显慢于Ad-hyrdC、Ad-null和正常对照组,差异有统计学意义,P=0.02;Ad-shyrdC组细胞的MTT值明显低于Ad-hyrdC、Ad-null和正常对照组,P=0.03;Ad-shyrdC组胃癌瘤体生长率较Ad-hyrdC、Ad-null组以及正常对照组明显减慢,P=0.02。结论:hyrdC基因具有促进胃癌细胞生长的功能,而沉默hyrdC基因可抑制胃癌细胞的生长,hyrdC基因有望成为胃癌基因治疗领域的新靶点。
Objective: To observe the effect of RNAi targeting silencing hyrdC gene on the growth of gastric cancer cell line SGC-7901. Methods: The recombinant adenovirus Ad-hyrdC and Ad-shyrdC carrying hyrdC gene and silencing hyrdC gene were successfully transfected into SGC-7901 gastric cancer cells. The growth of gastric cancer cells in each group was observed by trypan blue cell counting and MTT assay. The gastric cancer cells were inoculated subcutaneously in nude mice to construct nude mice tumor model to observe the growth of gastric cancer in each group. Results: Ad-shyrdC group was significantly slower than Ad-hyrdC, Ad-null and normal control group, the difference was statistically significant, P = 0.02; Ad-shyrdC group MTT value was significantly lower than Ad-hyrdC, Ad- null and normal control group, P = 0.03. The growth rate of gastric cancer in Ad-shyrdC group was significantly slower than Ad-hyrdC, Ad-null group and normal control group, P = 0.02. Conclusion: hyrdC gene can promote the growth of gastric cancer cells, and silence hyrdC gene can inhibit the growth of gastric cancer cells, hyrdC gene is expected to become a new target in the field of gene therapy of gastric cancer.