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利用已分离纯化的神经生长因子（ＮｅｒｖｅＧｒｏｗｔｈＦａｃｔｏｒ，ＮＧＦ）为抗原，制备兔抗ＮＧＦ抗血清，用饱和（ＮＨ４）２ＳＯ４盐析和ＰｒｏｔｅｉｎＡ亲和层析的方法，纯化其中的ＩｇＧ组分，再将其偶联到经ＣＮＢｒ活化的Ｓｅｐｈａｒｏｓｅ４Ｂ上，制成抗ＮＧＦＩｇＧ免疫亲和柱。应用此亲和柱可一步纯化蛇毒中的ＮＧＦ，大大简化了ＮＧＦ纯化工艺。
Rabbit anti-NGF antiserum was prepared by using Nerve Growth Factor (NGF) as an antigen. The IgG fraction was purified by saturated (NH4) 2SO4 salting-out and Protein A affinity chromatography, Coupled to CNBr activated Sepharose 4B, made anti-NGFIgG immunoaffinity column. The affinity column can be used to purify NGF from snake venom in one step, which greatly simplifies the NGF purification process.