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目的探讨中药复方安神方抗抑郁作用的机制。方法以慢性中等强度应激刺激建立大鼠抑郁模型,用强迫游泳试验进行行为学评分,用高效液相电化学检测方法检测大鼠脑干内单胺类神经递质的含量,观察模型大鼠给药前后的变化。结果抑郁模型大鼠第7天与第28天水中强迫游泳不动时间分别为(133.4±24.0)s、(179.7±35.3)s,正常组则为(127.9±31.3)s、(131.8±29.3)s,模型组显著高于正常组(P<0.05);脑干内5-HT、NE含量显著降低,模型组为(0.093±0.031)ng/mg、(0.146±0.038)ng/mg,正常组为(0.160±0.023)ng/mg、(0.237±0.049)ng/mg(P<0.05);安神方高剂量组第7天与第28天水中强迫游泳不动时间分别为(121.5±27.8)s、(145.1±24.2)s,与模型组比较显著降低(P<0.05),大鼠脑干内5-HT、NE含量分别为(0.151±0.021)ng/mg、(0.208±0.057)ng/mg,较正常组显著提高(P<0.05)。结论安神方具有抗抑郁作用,对中枢单胺类神经递质的调节作用是其疗效机制之一。
Objective To explore the anti-depression mechanism of traditional Chinese medicine Anshen Tablet. Methods The rats model of depression was established by chronic moderate-intensity stress stimulation. Behavioral scores were determined by forced swimming test. The content of monoamine neurotransmitters in rat brainstem was detected by high-performance liquid electrochemical detection. Changes before and after administration. Results The forced swimming time of the model rats on day 7 and day 28 was (133.4 ± 24.0) s and (179.7 ± 35.3) s respectively, while that of the normal group was (127.9 ± 31.3) s and (131.8 ± 29.3) s, the model group was significantly higher than that of the normal group (P <0.05). The content of 5-HT and NE in the brain stem were significantly decreased in the model group (0.093 ± 0.031) ng / mg and (0.146 ± 0.038) (0.160 ± 0.023) ng / mg and (0.237 ± 0.049) ng / mg respectively (P <0.05). The time of forced swimming in the high-dose Anshen Fang group was (121.5 ± 27.8) s on days 7 and 28 , And (145.1 ± 24.2) s, respectively, compared with the model group (P <0.05). The contents of 5-HT and NE in the rat brainstem were (0.151 ± 0.021) ng / , Significantly higher than the normal group (P <0.05). Conclusion Anshen prescription has antidepressant effect on central monoamine neurotransmitter regulation is one of the mechanisms of its efficacy.