:应用反相高效液相色谱法检测了肾舒冲剂水煎液中的小檗碱。样品经超声提取后 ,以 C1 8化学键合硅胶为固定相 ,乙腈 - 0 .0 4 mol/ L H3PO4(体积比为 4 2∶ 58)为流动相 ,用 34 9nm的波长定量检测。测定结果表明 ,小檗碱的质量浓度在 1.2～ 19.2 mg/ L范围内线性良好 ,最低检测限为 0 .6m g/ L,测定批内 ( n=5)及批间 ( n=5)相对标准偏差分别为 0 .6%～ 3.5%和 5.3%～ 6.5% ,回收率为 89.10 %～ 91.35%。 : Berberine in Shenshu decoction decoction was tested by RP-HPLC. After the sample was extracted by sonication, the mobile phase consisted of C1 8 chemically bonded silica as stationary phase, acetonitrile - 0.4 mol / L H3PO4 (volume ratio 4: 58), and quantified with a wavelength of 34 9 nm. The results showed that berberine had a good linearity in the range of 1.2-19.2 mg / L, the lowest detection limit was 0.6m g / L, and the intra-assay (n = 5) and inter- The standard deviations were 0.6% ~ 3.5% and 5.3% ~ 6.5%, respectively. The recoveries ranged from 89.10% to 91.35%.