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背景:淀粉样蛋白(β-amyloidprotein,β-AP)是老年斑的核心成分,它在脑内的沉积与阿尔茨海默病(Alzheimer’sAD)发病有着密切的关系。现代研究证实β-AP来源于淀粉样前体蛋白(β-amyloidprecursorprotein,β-APP),如果β-APP基因表达受到抑制,β-AP在脑内的沉积就会减少,阿尔茨海默病(Alzheimerdisease,AD)有可能得到有效防治。因此,很多国内外学者都在积极寻找β-APP基因表达抑制物,企图发现防治AD的有效药物。目的:研究海风藤对人类神经母细胞瘤细胞β-APP基因表达的影响。设计:药物(海风藤)干扰和非药物干扰对照;目的基因(β-APP)与结构基因(β-Actin)对照。材料:人类神经母细胞瘤细胞系列(SK-N-SH),海风藤。方法:应用细胞培养和反转录—聚合酶链反应(RT-PCR)观察海风藤不同浓度和不同时间对β-APPmRNA的影响。结果:海风藤选择性的抑制β-APP基因表达,这种抑制作用随时间的延长和浓度的增加而增强。结论:海风藤选择性抑制β-APP基因表达,为其防治AD提供了更加可靠的实验依据。
BACKGROUND: β-amyloid protein (β-AP) is a core component of senile plaques. Its deposition in the brain is closely related to the pathogenesis of Alzheimer’s AD. Modern studies have confirmed that β-AP is derived from β-amyloid precursor protein (β-APP). If β-APP gene expression is inhibited, β-AP deposition in the brain will be reduced, Alzheimer’s disease ( Alzheimerdisease, AD) may be effectively controlled. Therefore, many domestic and foreign scholars are actively looking for β-APP gene expression inhibitors in an attempt to find effective drugs for the prevention and treatment of AD. OBJECTIVE: To study the effect of seawardweed on the expression of β-APP gene in human neuroblastoma cells. Design: drug (Hyloba canadensis) interference and non-drug interference control; target gene (β-APP) vs. structural gene (β-Actin) control. Materials: Human neuroblastoma cell line (SK-N-SH), seaweed. METHODS: Cell culture and reverse transcription-polymerase chain reaction (RT-PCR) were used to observe the effects of different concentrations and time of Rhizoma Mandshurica on β-APP mRNA. RESULTS: Kaempferi selectively inhibited the expression of β-APP gene, and this inhibitory effect increased with time and concentration. Conclusion: The selective inhibition of β-APP gene expression by A. japonicum provides a more reliable experimental basis for prevention and treatment of AD.