【摘 要】
：
As a specific guanine nucleotide exchange factor of Rac 1,Tiam 1(T-lymphoma invasion and metastasis inducing protein 1)is involved in a number of cellular event
【机 构】
：
Department of Molecular Genetics,Shanghai Medical College,Fudan University & Key Laboratory of Molec
论文部分内容阅读
As a specific guanine nucleotide exchange factor of Rac 1,Tiam 1(T-lymphoma invasion and metastasis inducing protein 1)is involved in a number of cellular events,such as cytoskeleton reorganization,cell adhesion,and cell migration.Since Tiaml was implicated in the invasion and metastasis of T-lymphoma cells and breast tumor cells,we compared the expression level of Tiaml in two human giant-cell lung carcinoma cell strains with high or low metastasis potential,and found that Tiaml expression level in high-metastatic 95D cells was higher than that in low-metastatic 95C cells.To further confirm the role of Tiam1 in invasion and metastasis,we constructed the antisense Tiam1 expression plasmid(pcDNA3-antiTiam1),which was transfected into 95D cells.A stable transfected clone with decreased Tiaml expression was screened and selected for further research.Transwell assay showed that down-regulation of endogenous Tiam 1 by anti-Tiam1 can reduce the in vitro invasiveness of 95D cells.Our results suggested that Tiam 1signaling contributed to the invasion and metastasis of the humar giant-cell lung carcinoma cells.
其他文献
黄瓜是我国主要蔬菜作物之一,也是世界性重要蔬菜。由于人工选育及栽培种的杂优化,使黄瓜自然资源的多样性受到侵蚀,栽培品种的遗传背景变得更为狭窄。近年来,随着日光温室、
通过对茉莉3个栽培品种在干旱胁迫下的生理特性、光合作用和细胞超微结构的变化进行比较研究,结果表明,在相同胁迫程度和胁迫时间下,茉莉不同品种电解质渗漏率、丙二醛(MDA)和叶绿素(Chl)含量、过氧化氢酶(CAT)、过氧化物酶POD和超氧化物歧化酶(SOD)活性差异较大,随着胁迫程度的加大和胁迫时间的延长,其活性逐渐下降,与单瓣茉莉和多瓣茉莉相比较,双瓣茉莉MDA含量没有显著增加,CAT、POD和S
中国特色社会主义进入新时代,而乡村发展过程中的诸多问题开始逐步凸显,其中亟待解决的一点即乡村文化自信的缺失.乡村文化自信是增强国家文化软实力、构建国家文化自信的重
The t(8;21)translocation is one of the most frequent chromosome abnormalities in acute myeloid leukemia.This transtocation creates a fusion between the acute my
The modified Cry1Ac was expressed in transgenic tobacco plants.To allow secretion of the Cry1Ac protein into the intercellular space,the signal peptide sequence
香港职业教育特色鲜明、机制灵活,基于对香港职业教育的学习和实地参观访问,本文拟先通过介绍香港职业训练局相关情况,再以两个有代表性的职业教育视角,尝试予以本土化理解和
Nkx3.1 is a prostate-specific homeobox gene related strongly to prostate development andprostate cancer. To study its regulation of transcription, 1.06 kb 5 ′
LIM domain proteins are important regulators in cell growth, cell fate determination, cell differentiation and remodeling of the cell cytoskeleton by their inte
Shock waves were elicited by transient pressure disturbances, which could be used to treat musculoskeletal disorders. In present studies, we i. nvestigated whet
To develop target thrombolytic agents with fibrinolytic activity, antiplatelet aggregation activity and reduced immunogenicity, two staphylokinase variants cont