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目的人体活体行肺、肝、肾穿刺及实验用动物的肺、肝、肾组织制作免疫荧光切片、石蜡HE切片,用以明确疾病的性质以及做科研时动物试验的组织进行科学病理分析。方法我院患者的活检组织标本、试验室的动物组织标本取1/4及1/3用普通胶水代替OCT包埋剂,冰冻机下行冰冻切片用以制作直接免疫荧光染色。剩余标本组织在脱水机改进时间后进行脱水处理,行石蜡包埋切片做HE染色。结果组织荧光制片效果好,无背景着色,切片完整,组织结构清晰。组织石蜡HE切片染色胞浆分明,切片完整。两种制片镜下观察定位准确。结论采用改进后方法制片,节约资金,制片的阳性强度及阳性检出率与未改进前相比效果好,定位准确。
Objective The human living lung, liver and kidney puncture and experimental animal lung, liver and kidney tissue immunofluorescence section, paraffin HE sections, to determine the nature of the disease as well as animal research organizations to conduct scientific pathological analysis. Methods Biopsies from our hospital were collected from 1/4 and 1/3 of the animal tissue specimens in the laboratory and replaced by OCT embedding agents. The frozen sections of the freezing machine were used for direct immunofluorescence staining. The remaining specimens in the dehydration machine to improve the time for dehydration, paraffin-embedded sections for HE staining. Results The results of tissue fluorescence preparation were good, no background was colored, the slices were intact and the structure of tissue was clear. Tissue paraffin HE sections stained clear cytoplasm, section integrity. Two kinds of film microscope positioning and accurate positioning. Conclusion The improved method of preparation, saving money, the positive rate of production and positive detection rate compared with before the effect is not improved, accurate positioning.