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目的研究rSj26-Sj32-Immunogold-Dipstick试剂对慢性日本血吸虫病的诊断价值。方法用rSj26-Sj32融合蛋白和日本血吸虫成虫抗原(SjAWA)Immunogold-Dipstick法检测慢性日本血吸虫病患者血清,同时以华支睾吸虫病、卫氏并殖吸虫病、泡型棘球蚴病、囊性棘球蚴病、乙型肝炎、肺结核患者和健康人血清作为对照,比较两种抗原检测抗体效果的差异。结果该法检测慢性日本血吸虫病的敏感性和特异性分别为92.50%和97.67%,诊断该病的阳性预告值、阴性预告值及诊断效率分别为97.37%、93.33%和95.18%,并且与其他疾病患者血清均无交叉反应。结论 rSj26-Sj32-Immunogold-Dipstick试剂可用于慢性日本血吸虫病的免疫诊断。
Objective To study the diagnostic value of rSj26-Sj32-Immunogold-Dipstick reagent for chronic schistosomiasis japonica. Methods The serum of patients with chronic schistosomiasis japonica was detected by rSj26-Sj32 fusion protein and SjAWA Immunogold-Dipstick method. Meanwhile, the serum levels of Clonorchis sinensis, Paragonimus westermani, alveolar echinococcosis, Echinococcosis, hepatitis B, tuberculosis patients and healthy human serum as a control, the difference between the two antigens detection antibody effect. Results The sensitivity and specificity of the method for the detection of chronic schistosomiasis japonica were 92.50% and 97.67% respectively. The positive predictive value, negative predictive value and diagnostic efficiency of the method were 97.37%, 93.33% and 95.18%, respectively, Serum of patients with no cross-reaction. Conclusion rSj26-Sj32-Immunogold-Dipstick reagent can be used for immunological diagnosis of chronic schistosomiasis japonica.