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目的:研究不同剂量培高利特(pergolide)和左旋多巴(L-dopa)对MPP+诱导的PC12细胞的作用。方法:在细胞培养中用不同剂量的pergolide和L-dopa干预对MPP+所诱导的PC12细胞的影响。观察不同时点PC12细胞活力。酪氨酸羟化酶(TH)蛋白含量及细胞凋亡数。结果:0.01ìmol·L-1pergolid提高MPP+损害的PC12细胞的生长,细胞活力升高。0.01和0.1ìmol·L-1pergolid提高MPP+损害后的PC12细胞TH蛋白含量,减少该细胞的凋亡数。0.1ìmol·L-1L-dopa提高MPP+损害后PC12细胞的活力。随剂量递增细胞活力递量;当达1000ìmol·L-1L-dopa时细胞活力减少最明显。结论:提示小剂量pergolid对MPP+诱导的PC12细胞损害有一定的保护作用。高剂量则有一定的损伤。
Objective: To investigate the effects of pergolide and L-dopa at different doses on MPP + -induced PC12 cells. Methods: The effect of different doses of pergolide and L-dopa intervention on MPP + -induced PC12 cells in cell culture was studied. PC12 cell viability was observed at different time points. Tyrosine hydroxylase (TH) protein content and apoptosis. Results: 0.01μmol·L-1pergolid increased MPP + -deficited PC12 cells and increased cell viability. 0.01 and 0.1μmol·L-1pergolid increased THP content in PC12 cells after MPP + injury and decreased the number of apoptotic cells. 0.1μmol·L-1L-dopa increases the viability of PC12 cells after MPP + damage. The cell viability was increased with dose; the cell viability decreased most significantly when the concentration reached 1000 μmol·L-1 L-dopa. Conclusion: It suggests that low dose of pergolid can protect PC12 cells induced by MPP +. High doses have some damage.