在人癌性胸腹水中除含有肿瘤细胞外,还含有大量的淋巴细胞及其它组织细胞。这说明胸腹水中有许多能维持细胞生长,刺激细胞增殖的因素。在目前的组织培养中一般采用培养液中加10～20％的小牛血清,因血清来源不同,效果也差别较大,且价格较高。我们采用来源方便的临床病人的癌性 In addition to tumor cells, human cancerous hydrothorax and ascites also contain a large number of lymphocytes and other tissue cells. This shows that there are many factors in the chest and abdomen that can sustain cell growth and stimulate cell proliferation. In the current tissue culture, 10-20% calf serum is generally used in the culture fluid. Because of different serum sources, the effect is also very different, and the price is higher. We use the cancerous nature of clinical patients with convenient sources