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目的研究锌与氟联合作用对小鼠成釉细胞DNA损伤的影响。方法体外培养小鼠成釉细胞,分别设立对照组和0.25、0.50、1.00、2.00、4.00 mmol/L氟化钠单独染毒组及5.00、10.00、20.00μmol/L硫酸锌单独染毒组以及0.25、0.50、1.00、2.00、4.00 mmol/L氟化钠+5.00、10.00、20.00μmol/L硫酸锌联合染毒组。小鼠成釉细胞经硫酸锌预处理24 h后,再与氟化钠联合作用。染毒24 h后收获细胞,采用单细胞凝胶电泳(SCGE)检测DNA损伤情况。结果与对照组比较,氟化钠单独染毒小鼠成釉细胞的尾长、Olive尾矩、尾DNA%、尾长/头长值均明显升高(P<0.05),而硫酸锌单独染毒组以上指标均明显下降(P<0.05)。硫酸锌+氟化钠联合作用小鼠成釉细胞的尾长、Olive尾矩、尾DNA%、尾长/头长值低于对照组与相同剂量氟化钠单独染毒组(P<0.05)。与相同剂量氟化钠+10μmol/L硫酸锌联合染毒组比较,5.00和20.00μmol/L硫酸锌+氟化钠联合染毒组Olive尾矩均较高(P<0.05)。结论适量的锌对氟致小鼠成釉细胞DNA损伤有拮抗作用。
Objective To study the effects of zinc and fluorine on the DNA damage of ameloblasts in mice. Methods Mouse ameloblasts were cultured in vitro and control group and 0.25, 0.50, 1.00, 2.00, 4.00 mmol / L sodium fluoride alone and 5.00, 10.00, 20.00 μmol / L zinc sulfate alone and 0.25 , 0.50, 1.00, 2.00, 4.00 mmol / L sodium fluoride + 5.00,10.00,20.00μmol / L zinc sulfate combined exposure group. Mouse ameloblasts pretreated with zinc sulfate for 24 h, and then combined with sodium fluoride. The cells were harvested 24 hours after exposure to DNA damage by single cell gel electrophoresis (SCGE). Results Compared with the control group, tail length, Olive tail moment, tail DNA%, tail length / head length of ameloblasts were significantly increased (P <0.05) The above indicators were significantly decreased (P <0.05). The tail length, Olive tail moment, tail DNA%, tail length / head length of ameloblasts were lower than those of the control group and the same dose of sodium fluoride alone (P <0.05) . Olive tail moments of 5.00 and 20.00μmol / L zinc sulphate plus sodium fluoride combined exposure group were higher than those of the same dose sodium fluoride + 10μmol / L zinc sulphate combined exposure group (P <0.05). Conclusion An appropriate amount of zinc antagonizes the DNA damage induced by fluoride in ameloblasts of mice.