,Reactive oxygen species accumulation patterns of alfalfa root nodules identified using an optimized

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ROS include superoxide anion radical (O2-),hydrogen peroxide (H2O2),singlet oxygen (O.O),hydroxyl radical (OH.),and hydroxyl anion (OH-).O2-,the radical with the highest oxidation compacity to produce H2O2,OH.and OH-,is a byproduct of aerobic respiration in organisms.Rhizobia and their legume hosts are aerobic organisms,and nitrogen-fixing nodules formed on legume roots are developed from their symbiotic interactions.Therefore,ROS are generated in legume nodules through aerobic respiration by both symbiotic partners.
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