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以兔眼蓝莓茎尖和茎段形成的愈伤组织和长期继代培养的试管苗为材料,接种于改良的WPM和1/2WPM的培养基中,研究活性炭与Vc液对兔眼蓝莓外植体和愈伤组织形成中褐化的影响以及外源体、活性炭对无根苗瓶内生根的影响。结果表明,当培养基中加入Vc液并含100～200 mg·L-1的活性炭时,能有效防止外植体褐化的发生;当培养基中加入Vc液并含200～500 mg·L-1活性碳时,可有效防止愈伤组织褐化发生。蓝莓继代培养试管苗在1/2 WPM的培养基中添加0.2 mg·L-1IBA,并含100～200 mg·L-1活性炭时生根情况最为理想,经过7 d暗培养,30 d后生根率高达87.8%。
The callus formed from the shoot tip and stem of blue-eyed blueberry and the long-term subcultured in vitro were inoculated into modified WPM and 1 / 2WPM medium to study the effect of activated carbon and Vc solution Body and callus formation in the browning of the exogenous body and activated carbon on rooting in rootless seedling bottle. The results showed that the addition of Vc solution to the medium containing 100-200 mg · L-1 of activated carbon could effectively prevent browning of explants. When Vc solution was added to the medium and contained 200-500 mg · L -1 activated carbon, which can effectively prevent browning callus. In vitro culture of blueberry in vitro culture medium supplemented with 0.2 mg · L-1 IBA at 1/2 WPM medium with 100-200 mg · L -1 activated charcoal was most ideal. After 7 d dark culture, Rate up to 87.8%.