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目的探讨健康人和2型糖尿病(T2DM)患者血清游离脂肪酸(FFA)之间的相关关系,确定潜在生物标志物。方法采用气相-质谱联用(GC-MS)方法检测56例健康体检者(对照组)和57例2型糖尿病患者(2型糖尿病组)血清游离脂肪酸水平,结合PCA和PLS-DA方法对检测出的游离脂肪酸进行分析。结果 2型糖尿病组高密度脂蛋白(HDL-C)水平较对照组显著降低(P<0.05),葡萄糖(GLU)、血清甘油三酯(TG)、低密度脂蛋白(LDL-C)胆固醇(CHOL)水平显著升高(P<0.01);十二烷酸(C12:0)、棕榈酸(C16:0)、反-油酸(C18:1T)、亚油酸(C18:2)、花生酸(C20:0)、二十碳五烯酸(C20:5)、γ-亚麻酸(γ-C18:3)水平较对照组显著降低(P<0.05),硬脂酸(C18:0)、花生一烯酸(C20:1)、二十碳二烯酸(C20:2)、二十碳三烯酸(C20:3n-6)、花生四烯酸(C20:4)、山嵛酸(C22:0)、二十二碳五烯酸(C22:5n-3)、二十二碳六烯酸(C22:6ns)水平较对照组显著降低(P<0.01),其他各游离脂肪酸无显著性差异(P>0.05)。结论 2型糖尿病的发生与体内脂代谢紊乱有关,以C18:1、C18:2和C16:0作为2型糖尿病潜在生物标志物。
Objective To investigate the relationship between serum free fatty acid (FFA) and healthy volunteers in patients with type 2 diabetes mellitus (T2DM) and to identify potential biomarkers. Methods The levels of serum free fatty acids in 56 healthy subjects (control group) and 57 type 2 diabetic patients (type 2 diabetes mellitus group) were detected by gas chromatography-mass spectrometry (GC-MS). PCA and PLS-DA The free fatty acids were analyzed. Results Compared with the control group, the levels of HDL-C in type 2 diabetes group were significantly lower than those in control group (P <0.05). The levels of GLU, TG, LDL-C, (P <0.01); C12: 0, C16: 0, C18: 1T, C18: 2, peanut The levels of acid (C20: 0), eicosapentaenoic acid (C20: 5) and γ-linolenic acid (γ-C18: 3) were significantly lower than those of the control group (P < , Arachidonic acid (C20: 1), eicosadienoic acid (C20: 2), eicosatrienoic acid (C20: 3n-6), arachidonic acid (C20: 4), behenic acid (C22: 0), docosapentaenoic acid (C22: 5n-3) and docosahexaenoic acid (C22: 6ns) were significantly lower than those in the control group (P <0.01). The other free fatty acids Significant difference (P> 0.05). Conclusion The occurrence of type 2 diabetes is related to the disorder of lipid metabolism in vivo. C18: 1, C18: 2 and C16: 0 are potential biomarkers of type 2 diabetes.