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目的研究虾青素对对乙酰氨基酚所致小鼠肝损伤的保护作用及其作用机制。方法 40只小鼠随机分为5组(n=8):正常对照组、模型组和虾青素低、中、高剂量(50、125和250 mg.kg-1)组。各剂量组小鼠给予虾青素连续灌胃7 d后一次性腹腔注射对乙酰氨基酚400 mg.kg-1制备急性肝损伤模型,光镜观察肝脏的组织学改变,测定血清和肝组织的生化指标。结果与模型组比较,虾青素组血清中丙氨酸转氨酶、天冬氨酸转氨酶含量明显降低(P<0.01),肝组织中丙二醛含量降低,超氧化物歧化酶、谷胱甘肽过氧化物酶活性提高(P<0.01)。结论虾青素能增强小鼠体内酶防御系统功能,提高清除自由基的能力,对对乙酰氨基酚所致小鼠肝损伤具有明显保护作用。
Objective To study the protective effect of astaxanthin on paracetamol-induced liver injury in mice and its mechanism. Methods Forty mice were randomly divided into five groups (n = 8): normal control group, model group and astaxanthin low, medium and high doses (50, 125 and 250 mg.kg-1) groups. The mice in each dose group were given astaxanthin continuously for 7 days and then intraperitoneally injected with acetaminophen 400 mg.kg-1 to prepare the model of acute liver injury. The liver histological changes were observed with light microscope, and the serum and liver tissue Biochemical Indicators. Results Compared with the model group, the contents of alanine aminotransferase and aspartate aminotransferase in the astaxanthin group were significantly decreased (P <0.01) and the levels of malondialdehyde in the liver tissue were decreased. The levels of superoxide dismutase, glutathione Peroxidase activity increased (P <0.01). Conclusion Astaxanthin can enhance the function of the enzyme defense system in mice and improve the ability of scavenging free radicals. It has a significant protective effect on acetaminophen-induced liver injury in mice.