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本文用单克隆抗体荧光偏振免疫分析法(MAb-FPIA)与多克隆FPIA 法(PC-FPIA)测定肾移植病人环孢素A(CsA)的全血浓度并进行了比较。49例肾移植术后病人口服不同剂量CsA 期间,共监测CsA 全血谷浓度65例次。结果表明。在MAb-FPIA 法观察浓度40～600ng/ml 的范围内,PC-FPIA 法的测定结果为110～1700ng/ml,是MAb-FPIA 法的1.7～3.9倍。两种方法之间有良好的线性关系(r=0.9067,P<0.01)。按术后不同时间分组比较,M/P 比值(MAb-FPIA 法/PC-FPIA 法)随术后时间延长而减小,各组间的差异有显著意义(P<0.05)。
In this paper, monoclonal antibody fluorescent polarization immunoassay (MAb-FPIA) and polyclonal FPIA (PC-FPIA) were used to determine the concentration of CsA in renal transplant patients. 49 cases of kidney transplantation patients after oral administration of different doses of CsA, a total of 65 cases of CsA whole blood trough concentrations were monitored. The results show. The measurement result of the PC-FPIA method was 110 to 1700 ng / ml in the range of 40 to 600 ng / ml observed by the MAb-FPIA method, which was 1.7 to 3.9 times that of the MAb-FPIA method. There was a good linear relationship between the two methods (r = 0.9067, P <0.01). The M / P ratio (MAb-FPIA / PC-FPIA) decreased with the extension of time after operation. The differences among the groups were significant (P <0.05).