Drought is a major production constraint for major fruits and vegetable crops in the tropics. This study was conducted to investigate the effect of limited water supply at three growth stages(vegetative, flowering and fruiting) on the accumulation of proline and soluble sugars in three pepper species. Seeds of the three pepper species, Capsicum chinense Jacq., C. annuum L. and C. frutescens L. were raised in a nursery and the seedlings were transplanted into seventy two plastic pots arranged in a randomized complete block design with three replicates, 25 days after planting. Four water treatments, 200 m L of water supplied twice daily(W1), once in every three days(W2), once in every five days(W3), and zero water supplied throughout growing period(W0) were imposed at three vegetative, flowering, and fruiting growth stages. Data were collected on relative water content, free proline and total soluble sugar. Data collected were subjected to analysis of variance and means were separated using Duncan’s multiple range test. Results show that the concentration of proline and soluble sugar in leaves of the three pepper species were found to be remarkable at the different stages of growth in the stressed plants. Drought is a major production constraint for major fruits and vegetable crops in the tropics. This study was conducted to investigate the effect of limited water supply at three growth stages (vegetative, flowering and fruiting) on ​​the accumulation of proline and soluble sugars in three pepper Seeds of the three pepper species, Capsicum chinense Jacq., C. annuum L. and C. frutescens L. were raised in a nursery and the seedlings were transplanted into seventy two plastic pots arranged in a randomized complete block design with three replicates , 25 days after planting. Four water treatments, 200 m L of water supplied twice daily (W1), once in every three days (W2), once in every five days (W3), and zero water supplied throughout growing period (W0) were imposed at three vegetative, flowering, and fruiting growth stages. Data were collected on relative water content, free proline and total soluble sugar. Data collected were subjected to analysis of variance and means were separated us ing Duncan’s multiple range test. Results show that the concentration of proline and soluble sugar in leaves of the three pepper species were found to be remarkable at the different stages of growth in the stressed plants.