目的通过盆栽试验研究不同程度盐胁迫对乌拉尔甘草叶片亚显微结构的影响。方法以二年生乌拉尔甘草苗为材料,设置2个盐水平,分别为12 g/kg NaCl干土(Na_1)、15 g/kg(Na_2),同时设置对照组(CK),采用超薄切片技术制备样品,并于透射电子显微镜下观察细胞结构。结果发现对照组的叶片叶绿体结构紧实,膜系统完整;中度盐浓度下(12 g/kg NaCl)叶绿体内存在极少淀粉粒,并被大量的线粒体包围;高盐浓度下(15 g/kg NaCl)淀粉粒增多,波浪状细胞壁,叶绿体被膜严重损伤而降解,边缘结构遭到破坏,类囊体膨大,片层结构排列松散且片层间距加大,叶绿体降解呈离散状态,线粒体嵴膨大,部分出现空泡化现象。结论盐胁迫对乌拉尔甘草叶片细胞叶绿体、线粒体等的超微结构具有损伤作用,且盐浓度愈高损伤作用愈明显。 Objective To study the effects of salt stress on the submicroscopic structure of leaves in Ullaria licorice under different degrees of pot experiment. Methods Two-year-old Ural licorice seedlings were used as material to set up two salt levels, which were respectively 12 g / kg NaCl dry soil (Na_1) and 15 g / kg (Na_2), and the control group (CK) Samples were observed under a transmission electron microscope for cell structure. The results showed that the chloroplast structure of the control group was tight and the membrane system was intact. There were few starch granules in the chloroplast at 12 g / kg NaCl and were surrounded by a large number of mitochondria at the medium salt concentration (15 g / kg NaCl), the wavy cell wall and chloroplast were severely damaged and degraded, the marginal structure was destroyed, the thylakoid expanded, the lamellar structure was loose and the lamellar spacing was increased, the chloroplast degraded in a discrete state and the mitochondrial cristae were swollen , Part of the phenomenon of vacuoles. Conclusion Salt stress can damage the ultrastructure of chloroplast, mitochondria and so on in leaf cells of Ural licorice, and the higher the salt concentration, the more obvious the damage effect is.