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[目的]探讨丙型肝炎患者血清中丙型肝炎病毒核酸(HCV RNA)含量与丙型肝炎病毒抗体(抗-HCV)及丙氨酸氨基转移酶(ALT)间的相关性.[方法]采用实时荧光定量PCR检测302例丙型肝炎患者血清中HCV RNA含量,同时采用ELISA法检测抗-HCV水平,利用全自动生物化学检测仪测定ALT水平.[结果]302例丙型肝炎患者血清标本中,抗-HCV均呈阳性,其中199份标本HCV RNA含量高于检测上限(103 U/mL),2种检测方法的符合率为65.9%.ALT异常率随HCV RNA含量的增高而升高,两者呈显著正相关(r=0.155,P<0.05);而ALT水平变化与HCV RNA含量无相关性(r=0.001,P>0.05).[结论]HCV RNA含量与抗-HCV水平是诊断HCV感染的重要指标,HCV RNA含量结合ALT水平测定可帮助临床了解HCV在体内复制的状况及肝脏损伤情况.
[Objective] To investigate the correlation between the content of hepatitis C virus (HCV RNA) in serum of hepatitis C patients and hepatitis C virus antibody (anti-HCV) and alanine aminotransferase (ALT). [Methods] Real-time quantitative PCR was used to detect the serum HCV RNA in 302 patients with hepatitis C, and the level of anti-HCV was detected by ELISA, and the level of ALT was measured by automatic biochemical analyzer. [Results] Of the 302 patients with hepatitis C, , HCV-positive were all positive.The HCV RNA content in 199 samples was higher than the upper limit of detection (103 U / mL), and the coincidence rate of the two methods was 65.9% .The abnormal rate of ALT increased with the increase of HCV RNA, (R = 0.155, P <0.05). There was no correlation between ALT level and HCV RNA content (r = 0.001, P> 0.05). [Conclusion] HCV RNA content and anti-HCV level An important indicator of HCV infection, HCV RNA content combined with ALT levels can help clinical understanding of HCV replication in vivo and liver damage.