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目的建立山羊股骨远端骨洞模型,观察万古霉素缓释微球在模型体内释放药物的过程。方法山羊双侧股骨远端形成两个圆柱形骨洞,PMMA 封闭14 d 后,再次暴露骨洞,分别在右侧和左侧移植万古霉素微球和无万古霉素的微球。通过高效液相检测各部位的万古霉素浓度,并以葡萄球菌作为检测菌株,评估其抗菌活性。结果 X 线见骨洞约为2.5 cm×1.5 cm×1.5 cm,移植微球后右侧骨洞内的万古霉素浓度最高,1 d 达最高值(294.71±20.45)mg/L;左侧和血液中的浓度在2 d 达高峰,为(5.54±0.97)mg/L 和(29.98±2.61)mg/L;超过对葡萄球菌敏感折点值(5 mg/L)的持续释放天数分别为21、1、6 d。结论建立的山羊股骨远端骨洞模型适合于观察药物缓释载体在骨组织内释放药物的动态过程,便于检测洗脱出的药物浓度。
Objective To establish the distal femoral osteotomy model and to observe the release of vancomycin sustained-release microspheres in the model. Methods Two cylindrical femoral bones were formed in the distal femur of the bilateral femur. After 14 days of PMMA occlusion, the femoral holes were re-exposed. Vancomycin microspheres and vancomycin-free microspheres were transplanted to the right and left sides, respectively. The concentration of vancomycin in each part was detected by HPLC. Staphylococcus was used as the test strain to evaluate its antibacterial activity. Results X-ray showed a bone cavity of about 2.5 cm × 1.5 cm × 1.5 cm. The highest concentration of vancomycin was found in the right bone hole after transplantation of microspheres (294.71 ± 20.45 mg / L) The concentrations in blood reached the peak at (5.54 ± 0.97) mg / L and (29.98 ± 2.61) mg / L on the 2nd day, respectively. The number of days of sustained release beyond the sensitive point of exposure to Staphylococcus aureus (5 mg / L) , 1,6 d. Conclusion The model of distal hole in goat femur is suitable for observing the dynamic process of releasing drug in bone tissue of drug sustained-release carrier, and it is convenient to detect the drug concentration of eluted drug.