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目的通过对体检人群输血传播指标的检测,探究云南昆明地区第4、5组群输血传播病毒分子流行病学的特点。方法本次研究材料取于2016年4月在云南省第一人民医院体检科223份体检人群的血液标本,充分离心后提取血清,在零下80℃保存。利用聚合酶链式反应对血清输血传播病毒进行检测,阳性样品测序以后,对其同源性与系统进化予以分析。结果本研究对223份血清予以检测,并根据序列的测定与比对,明确了150份阳性标本,其病毒感染率为64.38%,第4组群检测出47份,其输血传播病毒的感染率21.08%;第5组群检测出77份,其输血传播病毒的感染率34.53%;混合感染9份,感染率4.04%。结论获取了本地区第4、5组群输血传播病毒流行病学的有关数据,对输血传播病毒具有预防作用。
Objective To detect the molecular epidemiology of transfusion transmitted viruses in groups 4,5 of Yunnan Kunming by detecting the blood transfusion index of medical examination population. Methods The materials of this study were collected from blood samples from 223 medical examinations of the First People’s Hospital of Yunnan Province in April 2016. After centrifugation, serum was extracted and stored at minus 80 ℃. The polymerase chain reaction (PCR) was used to detect the blood transfusion transmitted virus. After the positive samples were sequenced, their homology and phylogenetic analysis were analyzed. Results In this study, 223 serum samples were tested, and according to the sequence determination and comparison, 150 positive samples were identified, the virus infection rate was 64.38%, the fourth group was 47, the infection rate of transfusion transmitted virus 21.08%. In the fifth group, 77 samples were detected. The infection rate of transfusion transmitted virus was 34.53%. Nine were mixed infection with infection rate of 4.04%. Conclusion The epidemiological data of transfusion transmitted viruses in groups 4 and 5 of this region were obtained, which have a preventive effect on transfusion transmitted virus.