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目的:评价抗抑郁剂地昔帕明和氟西汀对5,7-二羟色胺损伤神经元的拮抗作用。方法:原代培养10d的大鼠海马和皮层细胞加入不同浓度的地昔帕明和氟西汀作用30min后,用5,7-二羟色胺600μmol·L~(-1)损伤4h,MTT法结合形态学观察判断药物的保护作用。结果:地昔帕明和氟西汀对神经元损伤具有显著拮抗作用,剂量范围分别为0.8-10μmol·L~(-1)和0.04-0.6μmol·L~(-1)。结论:地昔帕明和氟西汀对5,7-二羟色胺的神经元损伤具有显著拮抗作用,氟西汀效价高于地昔帕明(约一个数量级)。两种抗抑郁剂对神经元的保护作用可能是它们抗抑郁作用的机制之一。
OBJECTIVE: To evaluate the antagonism of antidepressants desipramine and fluoxetine on neurons injured by 5,7-dihydroxytryptamine. METHODS: Primary cultured 10 day hippocampal and cortical cells were exposed to different concentrations of desipramine and fluoxetine for 30 min, and then injured with 5, 7-dihydroxytryptamine 600 μmol·L -1 for 4 h. MTT assay Learn to judge the protective effect of drugs. RESULTS: Desipramine and fluoxetine had significant antagonistic effects on neuronal injury in the dose range of 0.8-10μmol·L -1 and 0.04-0.6μmol·L -1, respectively. CONCLUSIONS: Desipramine and fluoxetine have significant antagonistic effects on neuronal injury induced by 5,7-dihydroxytryptamine, and fluoxetine has higher potency than desipramine (about one order of magnitude). The protective effects of two antidepressants on neurons may be one of the mechanisms of their antidepressant effect.