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目的:比较结直肠癌组织与正常癌旁组织中microRNA-145(miR-145)的表达变化。方法:收集30例手术切除的结直肠癌组织和远离癌组织的正常结直肠黏膜组织。提取总RNA,microRNA的引物反转录后,用miR-145特异性引物做荧光定量PCR反应。结果:荧光定量PCR显示结直肠癌组织miR-145的表达量明显降低,癌组织中的表达量为正常癌旁组织的0.353倍,差异有统计学意义(P<0.05)。结论:结直肠癌组织中miR-145的表达低于正常组织,在结直肠癌中发挥抑癌基因的作用。
Objective: To compare the expression of microRNA-145 (miR-145) in colorectal cancer tissues and adjacent normal tissues. Methods: Thirty cases of resected colorectal cancer tissues and normal colorectal mucosa away from cancer tissues were collected. Total RNA was extracted. After reverse transcription of the microRNA primer, miR-145 specific primers were used for quantitative PCR. Results: Fluorescent quantitative PCR showed that the expression of miR-145 in colorectal cancer tissues was significantly lower than that in normal tissues (0.353-fold, P <0.05). Conclusion: The expression of miR-145 in colorectal cancer tissues is lower than that in normal tissues and plays a role of tumor suppressor gene in colorectal cancer.