目的建立中药材中有机氯和拟除虫菊酯类农药残留的测定方法。方法样品用超声波提取、Florisil-活性炭固相柱净化,用ECD检测器同时测定了12种有机氯和拟除虫菊酯类农药。结果所测定农药标准曲线的r为0.9959～0.9995,回收率为85.8%～109.8%。结论新建方法简便、快速、准确,可用于中药材中12种农药的含量测定。 Objective To establish a method for the determination of organochlorine and pyrethroid pesticide residues in Chinese herbal medicines. Methods The samples were extracted by ultrasonic wave and purified on a Florisil-activated carbon solid phase column. Twelve organochlorine and pyrethroid pesticides were simultaneously detected by ECD detector. Results The standard curves of pesticides were determined to be 0.9959 ~ 0.9995 and the recoveries ranged from 85.8% to 109.8%. Conclusion The new method is simple, rapid and accurate and can be used for the determination of 12 pesticides in traditional Chinese medicine.