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为探究昆虫天然产物斑蝥素(cantharitin,CTD)对鳞翅目昆虫小菜蛾Plutella xylostella(L.)的触杀机制,利用透射电子显微镜观察了该物质处理小菜蛾4龄幼虫体壁组织结构的变化。斑蝥素亚致死剂量0.15μg/头和半致死剂量0.3μg/头点滴处理试虫前胸背板,中毒试虫反映结果类似。处理12h后中毒试虫虫体发黑,陆续死亡。处理12h后,体壁细胞细胞核固缩并且边极化,线粒体扭曲,内脊模糊出现空泡化,线粒体和粗面内质网数量下降;处理24h后,体壁细胞出现的类似处理12h后的症状,且症状更为明显。结果表明CTD对小菜蛾体壁表皮层组织结构无显著破坏作用,但对体壁细胞有明显的致毒作用。
In order to explore the contact mechanism of insect natural product cantharitin (CTD) against Plutella xylostella (L.) Against the Lepidopteran insect Plutella xylostella (L.), The tissue structure of the 4th instar larvae of Plutella xylostella was observed by transmission electron microscopy. Suicide of cantharidin 0.15μg / head and semi-lethal dose of 0.3μg / head trickle insects thoracodorsal, poisoning insects reflect similar results. After treatment for 12h, the test insects became black and died one after another. After 12 hours of treatment, the somatic cells were pyknotic and marginally polarized, the mitochondria were twisted, the inner ridges were vacuolized, and the numbers of mitochondria and rough endoplasmic reticulum were decreased. After treated for 24 hours, the somatic cells appeared similar after 12h Symptoms, and the symptoms are more obvious. The results showed that CTD had no significant destructive effect on the tissue structure of the body wall epidermis of Plutella xylostella, but had obvious toxicogenic effects on the somatic cells.