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DHBV的感染与肝病的关系还不甚明了。本文用斑点杂交法,液闪法和光镜病理分别对92只从市场采购的家鸭进行研究发现:A组(绍兴麻鸭)46只中有28只DHBVDNA(+)(60.8%),B组(昆山麻鸭)18只中有7只DHBV DNA(+)(38.9%),二组比较(P>0.05)无显著性差异,C组(天津白鸭)28只无一只DHBV DNA阳性,说明南方鸭比天津鸭易感。同时用DHBVDNA探针与人HBV DNA阳性的血清行杂交和用HBVDNA探针与鸭DHBV DNA阳性的血清行杂交结果均为阴性,说明二者之基因序列无同源性。DNA聚合酶(DNA-p)的检测发现其阳性率为6.5%并同时伴有DHBV DNA(+),说明DNA-p的检测
The relationship between DHBV infection and liver disease is not clear. In this study, 92 domestic ducks purchased from the market were studied by dot blot hybridization, liquid scintillation and light microscopy. The results showed that 28 DHBVDNA (+) (60.8%) of group A (Shaoxing ducks) There were 7 DHBV DNA (+) (38.9%) out of 18 in Kunshan ducks. No significant difference was found between the two groups (P> 0.05) Description of southern duck than Tianjin duck susceptible. At the same time, DHBVDNA probe hybridized with human HBV DNA-positive serum and hybridized with HBVDNA probe and duck DHBV DNA-positive serum were negative, indicating that the two genes have no homology. DNA polymerase (DNA-p) test found a positive rate of 6.5% with DHBV DNA (+), indicating the detection of DNA-p